Abstract Cervical cancer is a leading cause of death among women. The endocervical adenocarcinoma (ECA) represents an aggressive and metastatic type of cancer with no effective treatment options currently available. We evaluated the antitumoral and anti-migratory effects of hypericin (HYP) encapsulated on Pluronic F127 (F127/HYP) photodynamic therapy (PDT) against a human cell line derived from invasive cervical adenocarcinoma (HeLa) compared to a human epithelial cell line (HaCaT). The phototoxicity and cytotoxicity of F127/HYP were evaluated by the following assays: colorimetric assay, MTT, cellular morphological changes by microscopy and long-term cytotoxicity by clonogenic assay. In addition, we performed fluorescence microscopy to analyze cell uptake and subcellular distribution of F127/HYP, cell death pathway and reactive oxygen species (ROS) production. The PDT mechanism was determined with sodium azide and D-mannitol and cell migration by wound-healing assay. The treatment with F127/HYP promoted a phototoxic result in the HeLa cells in a dose-dependent and selective form. Internalization of F127/HYP was observed mainly in the mitochondria, causing cell death by necrosis and ROS production especially by the type II PDT mechanism. Furthermore, F127/HYP reduced the long-term proliferation and migration capacity of HeLa cells. Overall, our results indicate a potentially application of F127/HYP micelles as a novel approach for PDT with HYP delivery to more specifically treat ECA.

Resumo Ações de promoção da saúde na terceira idade são de extrema relevância para a prevenção de doenças e agravos decorrentes do processo de envelhecimento. Nesse contexto, surgiu o projeto de intervenção comunitária “Em Comum-Idade”. O objetivo deste artigo foi avaliar o impacto das ações deste projeto nas medidas antropométricas e na aptidão física funcional dos participantes, ao final da participação nas intervenções. As variáveis analisadas foram: Índice de Massa Corporal, Perímetro da Cintura, Relação Cintura/Quadril, Relação Cintura/Estatura, força de membros inferiores, flexibilidade de membros inferiores e superiores e agilidade e equilíbrio dinâmico. A amostra foi composta por 82 participantes. A maioria era do sexo feminino, não tinha o primeiro grau completo e frequentou menos de 50% das atividades. Não houve mudanças significantes para os valores de medidas antropométricas entre as avaliações inicial e final dos participantes. Foi encontrada melhora significante para os valores de testes de aptidão física funcional entre aqueles que mais participaram das ações do projeto. Ressalta-se a importância de se manter ações de promoção da saúde por longos períodos de tempo a fim de se obter resultados que impactem positivamente as condições de saúde e a qualidade de vida de idosos.

Abstract Actions to promote health among the elderly are extremely important for the prevention of diseases and complications arising from the aging process. In this context, the “Em Comum-Idade” community intervention project emerged. The objective of this article was to evaluate the impact of the actions of this project on anthropometric measurements and functional physical fitness of the participants at the end of the interventions. The variables analyzed were: body mass index, waist circumference, waist hip ratio, waist height ratio, power of the lower members, flexibility of upper and lower members and agility and dynamic balance. The sample consisted of 82 participants. Most participants were female, had not completed elementary school and attended less than 50% of the activities. There were no significant changes in the values of anthropometric measurements between the initial and final evaluations of the participants. Significant improvement was found for the values of tests of functional physical fitness among those who attended most of the actions of the project. It is important to maintain health promotion actions for long periods of time in order to get results that positively impact the health status and quality of life of the elderly.

Resumo Ações de promoção da saúde na terceira idade são de extrema relevância para a prevenção de doenças e agravos decorrentes do processo de envelhecimento. Nesse contexto, surgiu o projeto de intervenção comunitária “Em Comum-Idade”. O objetivo deste artigo foi avaliar o impacto das ações deste projeto nas medidas antropométricas e na aptidão física funcional dos participantes, ao final da participação nas intervenções. As variáveis analisadas foram: Índice de Massa Corporal, Perímetro da Cintura, Relação Cintura/Quadril, Relação Cintura/Estatura, força de membros inferiores, flexibilidade de membros inferiores e superiores e agilidade e equilíbrio dinâmico. A amostra foi composta por 82 participantes. A maioria era do sexo feminino, não tinha o primeiro grau completo e frequentou menos de 50% das atividades. Não houve mudanças significantes para os valores de medidas antropométricas entre as avaliações inicial e final dos participantes. Foi encontrada melhora significante para os valores de testes de aptidão física funcional entre aqueles que mais participaram das ações do projeto. Ressalta-se a importância de se manter ações de promoção da saúde por longos períodos de tempo a fim de se obter resultados que impactem positivamente as condições de saúde e a qualidade de vida de idosos.

Abstract Actions to promote health among the elderly are extremely important for the prevention of diseases and complications arising from the aging process. In this context, the “Em Comum-Idade” community intervention project emerged. The objective of this article was to evaluate the impact of the actions of this project on anthropometric measurements and functional physical fitness of the participants at the end of the interventions. The variables analyzed were: body mass index, waist circumference, waist hip ratio, waist height ratio, power of the lower members, flexibility of upper and lower members and agility and dynamic balance. The sample consisted of 82 participants. Most participants were female, had not completed elementary school and attended less than 50% of the activities. There were no significant changes in the values of anthropometric measurements between the initial and final evaluations of the participants. Significant improvement was found for the values of tests of functional physical fitness among those who attended most of the actions of the project. It is important to maintain health promotion actions for long periods of time in order to get results that positively impact the health status and quality of life of the elderly.